Quantification

For accurate quantitative analysis of TLC / HPTLC separations, a scanner is used to perform densitometric evaluation, i.e. produce chromatograms from bands measured at the appropriate wavelengths.
Different modes may be required to obtain accurate and consistent results, such as absorption or fluorescence.
Calibration can be performed on pre-defined standard samples, making it possible to qualify and quantify unknown samples.
These instruments offer a wide range of possibilities:
-Recording of adsorption spectra at desired points to facilitate substance identification
Measurements using reference wavelengths (two-wavelength measurements) to reduce background noise.
-Multi-wavelength analysis, to detect the most suitable measurement wavelengths for chromatograms of unknown samples.
A versatile, user-friendly software package:
Proquant, for the day-to-day operation of the densitometric scanner
-SpectraCalc, a library of spectra for identifying the substances revealed
-Provalid, internal control software for validating densitometric data
-IQ/OQ for CD60 densitometer
The comprehensive, high-precision Bionis densitometric scanners are the most advanced instruments for characterising samples in R&D and quality control, in the pharmaceutical, cosmetics and chemical industries, and for characterising natural and plant products.